Vacuum and AP-MALDI tandem/multistage mass spectrometry for the structural characterization of protein allergens directly from latex surfaces and elderberry syrups
Martina Marchetti, Jasmin Hirschmann and Günter Allmaier
Institute for Chemical Technologies and Analytics, Vienna University of Technology,
Vienna, Austria 

Mass spectrometric analysis of natural and recombinant preparations of birch and grass pollen allergens
Erler AM¹, Wallner M¹, Susani M², Monsalve R³, Fiebig H⁴, Becker WM⁵, Didierlaurent A⁶, van Ree R⁷, Ferreira F¹, Briza P¹, and the CREATE consortium
¹University of Salzburg, Salzburg, Austria; ²Biomay, Produktionsgesellschaft, Vienna, Austria; ³ALK-Abelló, Biochemical Allergy Research, Hørsholm, Denmark; ⁴Allergopharma, Joachim Ganzer KG, Reinbeck, Germany; ⁵Forschungszentrum Borstel, Division of Biochemical and Molecular Allergology, Borstel, Germany; ⁶Stallergènes, Antony, France; ⁷Sanquin Research, Amsterdam, The Netherlands;

Plant cells as an efficient production system for the two major Dermatophagoides pteronyssinus allergens Der p 1 and Der p 2
Veronique Gomord, David Lienard, Olivia Tran Dinh, Erika van Oort, Laurence Van Overtvelt, Caroline Bonneau , Eric Wambre, Muriel Bardor, Alain Didier–Laurent, François Dorlahc de Borne, Rene Delon, Ronald van Ree, Philippe Moingeon, Loïc Faye
CNRS UMR 6037, Université de Rouen, IFRMP 23, Monst Saint Aignan, France

Recombinant high molecular weight grass allergens groups 4 and 13
A. Nandy, M. Wald, H. Kahlert, B. Weber, R. Suck, O. Cromwell, H. Fiebig
Allergopharma Joachim Ganzer KG, Research & Development, Reinbek, Germany 

Allergy to blackgram (Vigna mungo): possible role of the membrane-associated cysteine protease?
Fabrizio Guarneri, Claudio Guarneri
Istituto di Dermatologia, Policlinico “Gaetano Martino”, Università di Messina, Messina, Italy.

Sequence polymorphism of Ole e 1 allergen among olive tree cultivars. Biological and clinical implications
A.M. Hamman-Khalifa, A.J. Castro, A. Jiménez-López, M.I. Rodríguez-García, J.D. Alché
Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Granada. Spain.

1,3-β-glucanases,  relevant allergens in pollen
Mayte Villalba, Oscar Palomares, Joaquín Quiralte, Miguel Treviño, Marta Brüix, Rosalía Rodríguez
Dpto. Bioquímica/ fac. Quimicas/ucm, Ciudad Universitaria’ Madrid, Spain 

Mal d 1 silencing in apple – an update
Gilissen LJWJ,^1,2,4,5 Krath BRX,^3,4 Smulders MJM,^1,2,4 Eriksen FD,^3,4 Dragsted LO,^3,4 & Van de Weg WE,^1,2,4,5
1Allergy Consortium Wageningen, Wageningen University and Research Centre, Wageningen, The Netherlands; ²Plant Research International, Wageningen, The Netherlands; ³Danish Institute for Food and Veterinary Research, Soeborg, Denmark; ⁴ISAFRUIT (EC: 016279/T5.4.1); ⁵EU-SAFE (QLK1-CT-2000-01394)

Identification of potential peanut-lupine cross-reactive proteins by computer-assisted search for amino acid sequence homology and allergen motifs
Fabrizio Guarneri¹, Claudio Guarneri¹, Salvatore Benvenga^2,3
1Istituto di Dermatologia, Policlinico “Gaetano Martino”, Università di Messina, Messina, Italy; 2Sezione di Endocrinologia del Dipartimento Clinico Sperimentale di Medicina e Farmacologia, Università di Messina, Messina, Italy; 3Programma Infradipartimentale di Endocrinologia Molecolare Clinica, Azienda Ospedaliera Universitaria Policlinico Gaetano Martino, Messina, Italy 

Kiwellin: a novel allergen isolated from kiwi fruit
Maria Antonietta Ciardiello¹, Roberta Crescenzo¹, Vito Carratore¹, Anna Agnese Stanziola², Sergio Zofra³, Luigi Romano³, Laura Camardella¹, Maurizio Tamburrini¹
1Institute of Protein Biochemistry, CNR, Napoli, Italy; 2Clinica Malattie Respiratorie, University Federico II, Azienda Ospedaliera di Rilievo Nazionale e alta Specializzazione “V. Monaldi”, Napoli, Italy; 3    Laboratori di Analisi – Azienda Ospedaliera di Rilievo Nazionale e Alta Specializzazione “V. Monaldi”, Napoli, Italy 

Characterization and isolation of profilin as a major allergen in watermelon allergy
Bárbara Cases^1,2, Carlos Pastor^1,4, Marina Pérez-Gordo^1,2, Félix Gil³, Fernando Vivanco^1,3 and Javier Cuesta²
1Dept. Immunology. Fundación Jímenez Díaz. Madrid. Spain; 2Dept. Allergy, Fundación Jímenez Díaz. Madrid. Spain; 3Dept. Biochemistry. Universidad Complutense. Madrid. Spain; 4SIC Ingenieros S.L.L. Madrid. Spain. 

Proteomics of natural Bet v 1
Mutschlechner, S., Erler, A., Ferreira, F., and Briza, P.
University of Salzburg, Department of Molecular Biology, Salzburg, Austria 

Seven Bet v 1 genes in a single birch tree encode pollen-expressed isoforms which vary in IgE-reactivity. Consequences for sensitization and cross-reactivity
M.F. Schenk^1,2, G.D. Esselink², M.J.M. Smulders^1,2, L.J.W.J. Gilissen^1,2
1Allergy Consortium Wageningen, Wageningen University and Research Centre, Wageningen, The Netherlands; 2Plant Research International, Wageningen University and Research Centre, Wageningen, The Netherlands

Allergenic characterization of Bla g 7, German cockroach tropomyosin, using recombinant proteins
Kyoung Yong Jeong¹, Kwang Hyun Shin¹, Chein-Soo Hong², Tai-Soon Yong¹
1Dept. of Parasitology and Institute of Tropical Medicine, 2Dept. of Internal Medicine; Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea 

Blo t 11 Immunodominant Peptide Allergen Expression and Allergenicity
John Donnie A. Ramos¹,  Cheong Nge², Chua Kaw Yan²
1Research Center for the Natural Sciences, Department of Biological Sciences, College of Science, and Graduate School, University of Santo Tomas, Manila, Philippines; 2Department of Paediatrics, Faculty of Medicine, National University of Singapore, Republic of Singapore

Engineering egg white ovomucoid to modulate IgE-mediated allergic response and potential application for targeted immunotherapy
Yoshinori Mine, Prithy Rupa
Department of Food Science, University of Guelph, Guelph, Ontario, Canada

Recombinant house dust mite allergens elicit experimental asthma in mouse
VanderElst  Luc, de Halleux Sonia, Toussaint Gaelle, Carlier Vincent, Jacquemin Marc, Saint-Remy JMR
Catholic University of Leuven, Center for Molecular and Vascular Biology, Campus Gasthuisberg, Leuven, Belgium 

Probiotic Lactobacillus strains used as oral adjuvant affect on the antigen uptake and modulate the immune response to a co-administered vaccine antigen
Éva Gelencsér¹, András Nagy¹, Á. Baráth¹, A. Halász¹, Marianne Polgár^2
1Central Food Research Institute, Department of Food Safety, Unit of Biology, Hungary
²Madarasz Children's Hospital, Gastroenterological Centre, Budapest, Hungary 

Immunocytochemical localization by transmission electron microscopy of Cup a 3, a major allergen from Cupressus arizonica pollen grains
María Suárez-Cervera¹, Beatriz Sastre², Ana Vega-Maray¹, Rocio Moyá², Teresa Castells¹, Victoria del Pozo², Carlos  Lahoz², Juan A. Seoane-Camba^1
1Department of Botany, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain; ²Jimenez Díaz Foundation Capio, Madrid, Spain 

Mould physiology - the key to control mould allergen expression: An example of the expression of Alt a 1 in Alternaria alternata
Österberg M, Larsson A, Lehmann-Olsson B
Allergon AB R&D, Välinge, Sweden

Stress-derived cereal allergen molecules
Gyöngyi Hajós¹, E. Horváth-Szanics¹, T. Janáky², J. Pauk³, M. Polgár^4
1Central Food Research Institute, Budapest; ²University of Szeged, Szeged; ³Cereal Research Non-Profit Company, Szeged; ⁴Madarász Children Hospital, Madarász Budapest, Hungary 

Release mechanisms of Pla a 1 and Pla a 2 pollen allergens
María Suárez-Cervera¹, Juan A. Asturias², Ana Vega-Maray¹, Teresa Castells¹, Ignacio Ibarrola², M. Carmen Arilla², Juan A. Seoane-Camba^1
1Department of Botany, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain; ²Bial-Arístegui, R&D Department, Bilbao, Spain 

Efficient activation of human basophils using rDer p 2 allergen and humanized monoclonal Der p 2-specific recombinant IgE antibodies – Implications of antibody affinity
Jens Holm¹, Lars H. Christensen¹, Helene Henmar¹, Oscar Duffort², Caroline Bolwig¹, Kaare Lund¹
ALK-Abelló A/S, Research, Horsholm, Denmark1, ALK-Abelló A/S, Research, Madrid, Spain² 

A Single Recombinant Hybrid Molecule Consisting of the Major Timothy Grass Pollen Allergens allowed in vivo and in vitro diagnosis of grass pollen allergy
C. Metz-Favre¹, B. Linhart²,  A. Purohit¹, F. de Blay¹, R. Valenta2, G. Pauli¹
1Department of Respiratory Diseases, Hôpitaux Universitaires de Strasbourg, France; 2Department of Pathophysiology, Medical University of Vienna, Austria 

Molecule-based diagnosis of ragweed and mugwort pollen allergens
G. Gadermaier¹, N. Wopfner¹, M. Kasper¹, G. Regl¹, R. Lang², F. Aberger¹, T. Hawranek², F. Ferreira¹, M. Wallner^1
1University of Salzburg, Department of Molecular Biology, Salzburg, Austria; ²Paracelsus Private Medical University Salzburg, Department of Dermatology, Salzburg, Austria

Short and giant ragweed are not allergenically identical
Riccardo Asero
Ambulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano (MI), Italy

Use of individual allergenic molecules to improve the diagnosis of hymenoptera allergy.
Monsalve R.I.¹, Gutiérrez R. ¹, Polo F. ¹, Lombardero M. ¹, Galán A. ¹, King T.P.², Marqués L.³, Barber D^1
1ALK-ABELLO, Research Department, Madrid, Spain; 2Rockefeller University, New York, United States; 3SEAIC Hymenoptera Committee, Lleida, Spain

Sensitization to mite allergens in Polish workers of a Zoo
Piotr Szilman¹, Ewa Szilman¹, Krzysztof Solarz¹, Aleksander L. Sieroń³, Hanna Maniurka¹, Magdalena Szilman², Elżbieta Meszyńska², Ewelina Cichecka^1
1Department of Parasitology, Medical University of Silesia, Sosnowiec, Poland; ²Department of Toxicology, Medical University of Silesia, Sosnowiec, Poland; ³Department of General and Molecular Biology and Genetics, Medical University of Silesia, Katowice, Poland

Predictive value of skin prick-tests using recombinant allergens for diagnosis of peanut allergy
Catherine Astier¹, Martine Morisset^1,2, Olivier Roitel¹, Fanny Codreanu^1,2, Sandrine Jacquenet¹, Patricia Franck^1,3, Virginie Ogier¹, Nicolas Petit^1,2, Barbara Proust¹, Denise-Anne Moneret-Vautrin^1,2, Wesley A. Burks⁴, Bernard Bihain¹, Hugh A. Sampson5, Gisèle Kanny^1,2
1EA3999 Allergic Diseases: Diagnosis and Therapeutics Vandoeuvre-les-Nancy, France; ²Department of Internal Medicine, Clinical Immunology and Allergology, University Hospital, Nancy, France; ³Biochemistry Laboratory, University Hospital, Nancy, France; ⁴Department of Pediatrics, Division of Pediatric Allergy and Immunology, Duke University Medical Center, Durham, NC, USA; ⁵Department of Pediatrics, Division of Allergy and Immunology, Mount Sinai Hospital, New York, NY, USA

Immediate Hypersensitivity to Leaf Extracts of Callisia fragrans (Inch plant) in a Dog
Susan E. Lee¹, Kenneth V. Mason^2
1School of Biomolecular and Biomedical Science, Griffith University, Nathan, Queensland, Australia; ²School Of Veterinary Science, University of Queensland, St Lucia and the Animal Allergy and Dermatology Service, Slacks Creek, Queensland, Australia

Association of IgE reactivity and allergic symptoms in a cross-sectional study of seafood processing workers
M Jeebhay¹, T Robins², I Swoboda³, R Baatjies⁴, N Balic³, S Spitzauer³, A L Lopata^5
1University of Cape Town, Occupational and Environmental Health Research Unit; School of Public Health and Family Medicine, Cape Town, South Africa; ²University of Michigan, Department of Environmental Health Sciences, Michigan, United States; ³Medical University of Vienna, Institute of Medical and Chemical Laboratory Diagnostics, AKH, Vienna, Austria; ⁴University of Cape Town, Occupational and Environmental Health Research Unit, School of Public Health and Family Medicine, Cape Town, South Africa; ⁵University of Cape Town, Division of Immunology, Faculty of Health Sciences, NHLS, Cape Town, South Africa

Screening allergic subjects with a panel of pollen allergens from birch, timothy grass, and pellitory
Lia Pirrotta, Enrico Scala, Emma Guerra, Maria Locanto, Chiara Mondino, Donato Quaratino, Alessandra Zaffiro, Mauro Giani, Adriano Mari
Center for Clinical and Experimental Allergy, IDI-IRCCS, Rome, Italy

Specific Immunotherapy Stimulates a Monocyte-Dependent and B7-H1/IL-10-Mediated Suppressive Loop Controlling Allergic Inflammation
Stefania Piconi¹, Daria Trabattoni², Marina Saresella³, Enrico Iemoli¹, Monica Schenal², Manuela Borelli², Ambra Mascheri², Alessandra Fusi1, Mario Clerici^2
1Allergy and Clinical Immunology Unit, Luigi Sacco Hospital, Milano; ²Chair of Immunology DISP LITA Vialba, University of Milano, Milano; and ³Laboratory of Biology, Don Gnocchi Foundation, IRCCS, Milano, Italy.

Sublingual immunotherapy for food allergy
Ernesto Enrique¹, Fernando Pineda², Tamim Malek¹, Joan Bartra³, Anna Cisteró-Bahíma^4
1Allergy Department, Hospital General de Castellón, Spain. ²I+D Department, Laboratorios Diater, Madrid, Spain. ³Allergy Department, Hospital General de Girona Dr Joseph Trueta, Girona, Spain. ⁴Allergy Department, Institut Universitari Dexeus, Universitat Autònoma de Barcelona, Barcelona, Spain.